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In addition, we used immunofluorescence and western blotting to detect the inflammatory cytokines in podocytes and found that the protein levels of IL-6, TNF-α, IL-1β and iNOS were elevated and the TGF-β1 level was downregulated in the HG group. Furthermore, podocytes were pretreated with the calpain inhibitor ALLM, and the protein levels of IL-6, TNF-α, IL-1β and iNOS were significantly reduced, while TGF-β1 was significantly increased. This finding suggested that the calpain inhibitor improves the damage to podocytes induced by hyperglycemia and inhibits the release of inflammatory factors. In conclusion, the present study revealed that the calpain inhibitor has a protective effect on podocytes in the rats with DN, which could be attributed to inhibition of protein aggregation and inflammation. The mechanism by which the calpain inhibitor reduces podocyte protein aggregation was related to the down-regulation of the protein expression levels of calpain-1 and calpain-2, and the up-regulation of the protein expression of TGF-β1. Therefore, the inhibition of calpain might provide a new strategy for the treatment of podocyte injury caused by DN. **Funding:** This research was supported by the Medical Scientific Research Foundation of Guangdong Province, No. B2015088; the Second Batch of Traditional Chinese Medicine Research of Guangdong Province, No. 2013049; the Natural Science Foundation of Guangdong Province of China, No. 2017A030313081. **Availability of data and materials:** What's New in the? Q: Select data from varchar column in Mysql I have a table in my mysql database with a column of varchar type. I have searched the internet but I can't seem to find a solution to query rows in that column. 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